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Optical microscope for the detection of fungal specimens
Release date: 2019/2/24 10:28:18 Click volume: 1595
Microscopic examination methods for several commonly used fungal specimens
Material processing
1. Overall examination of the tissue A small piece of the diseased tissue or the tissue tissue was cut out, and the pathogen on the surface of the tissue was observed with a low power microscope. This way you can see the state in which the germs are naturally born. If you can use the concentrated strong light on the surface of the specimen, you can see more clearly. Peel microscopy can sometimes be stripped.
2. Organize the whole inspection after entering the Ming Dynasty In order to observe the pathogen inside the tissue, the tissue can be transparent and then examined as a whole. There are many methods for transparency, and different methods can be used to test different methods according to different materials. Here, only the commonly used chloral hydrate transparent method is mentioned. The small pieces of leaves were fixed in 95% alcohol and an equal amount of glacial acetic acid stick solution for 24 hours, and then immersed in a saturated aqueous solution of chloral hydrate (5 g of chloral hydrate, 2 ml of water), after the tissue was transparent The extract was washed with water, stained with an aqueous solution of dilute aniline blue, and then capped with glycerin.
The chloral hydrate transparent method can also be used for pathogens that overwinter on the surface of the dead leaves and inside. The method is to immerse the dead leaves in a saturated solution of chloral hydrate overnight (to be transparent), rinse them with water several times, and then immerse them in a 10% potassium hydroxide aqueous solution for several days to remove the color of the dead leaves.
After rinsing with water several times, it is washed twice in 95% alcohol, immersed in absolute alcohol for three hours (alcohol to be replaced) to remove water, mixed with phenol turpentine (melted crystalline phenol 40 ml and 60 ml turpentine) Mixed) transparent, then washed with xylene, sealed with Canadian gum. If it is not permanently sealed, it can be directly examined by microscopy after transparency.
Material processing
1. Overall examination of the tissue A small piece of the diseased tissue or the tissue tissue was cut out, and the pathogen on the surface of the tissue was observed with a low power microscope. This way you can see the state in which the germs are naturally born. If you can use the concentrated strong light on the surface of the specimen, you can see more clearly. Peel microscopy can sometimes be stripped.
2. Organize the whole inspection after entering the Ming Dynasty In order to observe the pathogen inside the tissue, the tissue can be transparent and then examined as a whole. There are many methods for transparency, and different methods can be used to test different methods according to different materials. Here, only the commonly used chloral hydrate transparent method is mentioned. The small pieces of leaves were fixed in 95% alcohol and an equal amount of glacial acetic acid stick solution for 24 hours, and then immersed in a saturated aqueous solution of chloral hydrate (5 g of chloral hydrate, 2 ml of water), after the tissue was transparent The extract was washed with water, stained with an aqueous solution of dilute aniline blue, and then capped with glycerin.
The chloral hydrate transparent method can also be used for pathogens that overwinter on the surface of the dead leaves and inside. The method is to immerse the dead leaves in a saturated solution of chloral hydrate overnight (to be transparent), rinse them with water several times, and then immerse them in a 10% potassium hydroxide aqueous solution for several days to remove the color of the dead leaves.
After rinsing with water several times, it is washed twice in 95% alcohol, immersed in absolute alcohol for three hours (alcohol to be replaced) to remove water, mixed with phenol turpentine (melted crystalline phenol 40 ml and 60 ml turpentine) Mixed) transparent, then washed with xylene, sealed with Canadian gum. If it is not permanently sealed, it can be directly examined by microscopy after transparency.
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